Tuesday, December 24, 2013

A Pulse Chase Experiment

Part A

Black spots would appear on the exterior of the cell after 3 hours, indicating that the proteins had been secreted from the cell.
Part B

Proteins are synthesized in the rough ER, modified in the Golgi apparatus, and carried in secretory vesicles to the plasma membrane, where they are secreted.
Part C

This conclusion can be drawn from the results, since the radioactive spots were last detected at the lysosome, which is not a secretory vesicle.
Part D

Pulse–chase experiments allow for the tracking of molecules in a cell over time.
Part E

During the chase phase, cells are provided with a large amount of nonradioactive material, so they are notlabeled.
Part F

Cells are first "pulsed" with a large amount of labeled material for a short time.

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